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Releases: hisplan/sharp

v0.1.1

19 May 00:35
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  • Fixed the problem where one vs. rest plots fail if negative exists.
  • Other minor updates.

v0.1.0

30 Mar 20:25
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  • Adds automated basic QC notebook for Cell Hashing (aka. hashtag) and CITE-SEQ.
  • Disables read_from_cache, enables write_to_cache by default.

v0.0.13

03 Nov 15:41
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  • Added missing submit-cellplex.sh.
  • Added absolute minimum tutorial notebook.

v0.0.12

01 Sep 01:30
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  • Added proper handle for antibody name composed of just numbers.
  • Added outputs overview document.
  • Updated QC notebooks
  • Updated job templates
  • Removed deprecated code

v0.0.11

05 Aug 11:40
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v0.0.10

02 Aug 22:40
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  • Handles an edge case where doublets don't exist.
  • Refactored for SCING.
  • Uses seqc-utils:0.5.1.

v0.0.9

31 Jul 20:21
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  • Supports ASAP-seq.
  • Generates AnnData for all assays.
  • Updates the inspection scripts to use the generated AnnData.
  • Changes the workflow name from Sharp to Hashtag
  • Uses hto_gex_mapper to speed up the HTO <--> GEX translation process.
  • Adds maxRetries: 3 to the workflow option file.
  • Removed unnecessary input variables from CiteSeq and Preprocess.
  • Adds unit tests

v0.0.8

06 Jul 20:11
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  • Refactored to support Amazon ECR.
  • Renamed QC to SanityCheck.
  • Removed sc.pp.calculate_qc_metrics from CITE-seq AnnData.

v0.0.7

30 Jun 14:18
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  • Added demuxMode for K-Means based demultiplexing: 1=default, 2=noisy methanol, 3=aggressively rescue from doublets
  • Added minCount: total count for CB less than this threshold will be marked as negative (unreliable observations)
  • Beta support for 10x Cell Multiplexing (aka. cellplex)

v0.0.6

22 Jun 16:54
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  • Auto-computes memory requirements if -1 is passed to Sharp.resourceSpec. This helps process 400M+ reads and/or 20K+ cells
  • Refactored to support different versions of CITE-seq-Count.