Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Definition clarifications by @phillipeloher #1

Open
lpantano opened this issue Jul 16, 2018 · 1 comment
Open

Definition clarifications by @phillipeloher #1

lpantano opened this issue Jul 16, 2018 · 1 comment

Comments

@lpantano
Copy link
Contributor

Not sure if opening an issue here is appropriate or if there is a better place to put these questions. No real issues to report in this case, just some clarification/documentation questions.

header:

what is 'source ontology', example?
for 'sample names used in attribute' - does this include items such as SRA accessions?
'small RNA GFF version ' - is that the format of this specification? Or is this a different version?
Filter tags, really like the concept. Think we should give an example that's not just Pass/Fail. Such as CAUTION(ambiguous sequence, may not be an isomiR)
Attributes:

Read Name - is this optional when the attribute->expression flags are used?
Parent - is this precursor name the same as Column 1 precursor name? If so, is it redundant?
Hits - what is defined as a 'hit'? Is this different than Attributes->Expression?
Variant - what happens it multiple variants for the same isomiR? Will open up a different issue on this since it's more involved

@lpantano
Copy link
Contributor Author

  • we have examples here for : https://github.com/miRTop/mirGFF3/blob/master/definition.md, if the database is in a official resource then we use that, if not the URL of the database. That was something the parent GFF format had, but still is not implemented.

  • for sample names, is whatever the user wants to use actually, we can add more information for sure.

  • yeah, I would love to add official TAGS: I'll open an issue for this to see what people offer.

  • Read name, is optional, you can add it for debugging reasons

  • Parent can be redundant if the tool mapped against precursor, but if it was mapped to genomics, or translate to genomic coordinates at some point, then is the chromosome name.

  • Hits means to say the number of time the read is present in the GFF, for instance for ambigous reads.

  • Thanks for opening a new issue with the variant issue!

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

1 participant